scf (R&D Systems)

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R&D Systems scf
Scf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+epo/Recombinant+Human+Erythropoietin%2FEPO+(Tissue+Culture+Grade)/pm41991719-41-40-41
Average 94 stars, based on 101 article reviews

scf - by Bioz Stars, 2026-09

94/100 stars

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Cell Culture:

Article Title: ACK1 and BRK non-receptor tyrosine kinase deficiencies are associated with familial systemic lupus and involved in efferocytosis
Article Snippet: .. Briefly, PBMCs were cultured in QBSF-60 media supplemented with L-Asorbic Acid (50 μg/mL), human SCF (50 ng/mL; R&D; 255-SC-010/CF), human IL-3 (10 ng/mL; Peprotech; 200–03), human EPO (2 U/mL; R&D; 287-TC-500), IGF-1 (40 ng/mL; R&D; 291-G1-200), and Dexamethasone (1 μM; Sigma; D8893-1MG) for 9–12 days to expand the erythroblast population. .. Then 4 Sendai viral vectors (Thermo Fisher Scientific; A16517) expressing Oct3/4, Sox2, Klf4, or c-Myc are used for transduction of 2.5x10 5 cells with 10 MOI for each virus for 24 hr.

Article Title: ACK1 and BRK non-receptor tyrosine kinase deficiencies are associated with familial systemic lupus and involved in efferocytosis
Article Snippet: .. Briefly, PBMCs were cultured in QBSF-60 media supplemented with L-Asorbic Acid (50 μg/mL), human SCF (50 ng/mL) (R&D; 255-SC-010/CF), human IL-3 (10 ng/mL) (Peprotech; 200-03), human EPO (2 U/mL) (R&D; 287-TC-500), IGF-1 (40 ng/mL) (R&D; 291-G1-200), and Dexamethasone (1 μM) (Sigma; D8893-1MG) for 9 to 12 days to expand the erythroblast population. .. Then 4 Sendai viral vectors (ThermoFisher Scientific; A16517) expressing Oct3/4, Sox2, Klf4, or c-Myc are used for transduction of 2.5×10 5 cells with 10 MOI for each virus for 24 hr.

Recombinant:

Article Title: Erythropoietin receptor signal is crucial for periodontal ligament stem cell-based tissue reconstruction in periodontal disease.
Article Snippet: Functional knockdown and pharmacological activation of EPOR in PDLSCs Cont-PDLSCs were treated for three days with siEPOR and siCONT (20 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) using Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s instructions and used as siEPOR-PDLSCs and siCONT-PDLSCs. .. PD-PDLSCs were treated with recombinant human EPO (0.1 U/mL; R&D Systems, Minneapolis, MN, USA) or MOCK (PBS) for 2 days and used as EPOPD-PDLSCs and MOCK-PD-PDLSCs. ..

Article Title: Erythropoietin receptor signal is crucial for periodontal ligament stem cell-based tissue reconstruction in periodontal disease
Article Snippet: Cont-PDLSCs were treated for three days with siEPOR and siCONT (20 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) using Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s instructions and used as siEPOR-PDLSCs and siCONT-PDLSCs. .. PD-PDLSCs were treated with recombinant human EPO (0.1 U/mL; R&D Systems, Minneapolis, MN, USA) or MOCK (PBS) for 2 days and used as EPO-PD-PDLSCs and MOCK-PD-PDLSCs. ..

Control:

Article Title: Kidney Mesenchymal Stem Cell-derived Extracellular Vesicles Engineered to Express Erythropoietin Improve Renal Anemia in Mice with Chronic Kidney Disease.
Article Snippet: Extracellular vesicles (EVs) shed from kidney mesenchymal stem cells (KMSCs) show protective effects against acute kidney injury and progressive kidney fibrosis via mRNA transfer.. Previous studies report improvement of renal anemia following administration of genetically modified MSCs or peritoneal mesothelial cells that secrete erythropoietin (EPO).. Here, we determined whether EPO-secreting KMSC-derived EVs (EPO-EVs) can improve renal anemia in mouse models of chronic kidney disease (CKD).

Enzyme-linked Immunosorbent Assay:

Article Title: Delivery and expression of mRNA therapeutics during ex vivo kidney perfusion: A feasibility study in porcine and human donor kidneys
Article Snippet: During NMP, glucose and pH were adjusted if glucose dropped under 5 mmol and pH under 7.35 by adding a bolus of glucose 5% (50 mg/mL, Fresenius Kabi, 7340022100429) or sodium bicarbonate 8.4% (8.4 g/mL, Fresenius Kabi, 03058282) directly to the organ chamber. .. Human EPO in OK cell cultures was quantified by an EPO DuoSet ELISA ( R&D Systems, DY286-05) according to the manufacturer’s protocols. .. In total, 25 mg of kidney tissue was homogenized in M tubes (Miltenyi Biotec,130093236) in 500 μL of Pierce RIPA buffer (Thermo Scientific, 89901) supplemented with cOmplete Mini protease inhibitor cocktail (Merck KGaA, 11836153001).

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